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Fig. 5 | Biological Research

Fig. 5

From: ROS play an important role in ATPR inducing differentiation and inhibiting proliferation of leukemia cells by regulating the PTEN/PI3K/AKT signaling pathway

Fig. 5

ATPR regulates the PTEN/PI3K/Akt pathway in human NB4 and THP-1 cells. a NB4 cells were treated with an ATPR concentration gradient (10−5–10−9 M) for 72 h. Then, the protein expression of PTEN, AKT, p-AKT and PI3K was assessed by western blot. b NB4 cells were treated with ATPR (10−6 M) for different durations (0–72 h). Then, the protein expression of PTEN, AKT, p-AKT and PI3K was assessed by western blot. c THP-1 cells were treated with an ATPR concentration gradient (10−5–10−9 M) for 72 h. Then, the protein expression of PTEN, AKT, p-AKT and PI3K was assessed by western blot. d THP-1 cells were treated with ATPR (10−6 M) for different durations (0–72 h). Then, the protein expression of PTEN, AKT, p-AKT and PI3K was assessed by western blot. e NB4 cells were treated with ATPR (10−6 M) for 24 h and 72 h. Immunofluorescence was used to detect p-Akt. *p < 0.05, **p < 0.01 versus control group

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