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Fig. 3 | Biological Research

Fig. 3

From: ROS play an important role in ATPR inducing differentiation and inhibiting proliferation of leukemia cells by regulating the PTEN/PI3K/AKT signaling pathway

Fig. 3

ATPR triggers cell cycle arrest at G0/G1 phase in human NB4 and THP-1 cells. a NB4 cells were treated with ATPR (10−6 M) for different durations (0–72 h). The cell cycle distribution was analyzed by flow cytometry. b NB4 cells were treated with an ATPR concentration gradient (10−5–10−9 M) for 72 h. Then, the protein expression of cyclin A2, CDK4, cyclin D3 and p-Rb was assessed by western blot. c NB4 cells were treated with ATPR (10−6 M) for different duration (0–72 h). Then, the protein expression of cyclin A2, CDK4, cyclin D3 and p-Rb was assessed by western blot. d THP-1 cells were treated with ATPR (10−6 M) for different durations (0–72 h). The cell cycle distribution was analyzed by flow cytometry. e THP-1 cells were treated with an ATPR concentration gradient (10−5–10−9 M) for 72 h. Then, the protein expression of cyclin A2, CDK4, cyclin D3 and p-Rb was assessed by western blot. f THP-1 cells were treated with ATPR (10−6 M) at different time points (0–72 h). Then, the protein expression of cyclin A2, CDK4, cyclin D3 and p-Rb was assessed by western blot. *p < 0.05, **p < 0.01 versus control group

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