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Fig. 6 | Biological Research

Fig. 6

From: PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer

Fig. 6

PELI3 mediates the effects of miR-365a-5p on NSCLC cells proliferation, migration and invasion. a, b The expression levels of PELI3 were measured by qRT-PCR and western blot in A549 cells co-transfected with nothing (Mock), negative control of miRNA inhibitor and negative control of siRNA (NC-inhibitor + si-NC), miR-365a-5p inhibitor and negative control of siRNA (miR-365a-5p-inhibitor + si-NC) or miR-365a-5p inhibitor and PELI3 siRNA (miR-365a-5p-inhibitor + si-PELI3). c, d MTT and colony formation assays showed cell proliferation advantage induced by miR-365a-5p inhibitor was attenuated by knockdown of PELI3 in A549 cells. e Wound-healing assays showed the increased cell migration due to miR-365a-5p inhibitor was attenuated by knockdown of PELI3 in A549 cells. f Transwell assays showed the increased cell invasion due to miR-365a-5p inhibitor was attenuated by knockdown of PELI3 in A549 cells. The data represent the mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. (two-way ANOVA for c, student’s t-test for others)

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