Fig. 3
From: PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs40659-019-0230-y/MediaObjects/40659_2019_230_Fig3_HTML.png)
Knockdown of PELI3 inhibits tumor growth and metastasis. a, b Knockdown efficiency of PELI3 in A549 cells stably transfected with PELI3 shRNA (sh-PELI3-1 and sh-PELI3-2) or empty vector (sh-CTR) was confirmed by qRT-PCR and western blot. c, d Tumor growth curve of A549-sh-CTR and A549-sh-PELI3 cells implanted into the flank of male nude mice. e, f H&E staining of the metastatic nodules in the lung of A549 cells which stably transfected with PELI3 shRNA (sh-PELI3-1 and sh-PELI3-2) or empty vector (sh-CTR) following tail vein injection into nude mice (200 × scale bars) and incidence of lung metastasis in mice following tail vein injection of the respective A549 cells. *P < 0.05; **P < 0.01; ***P < 0.001