Fig. 4

TUSC2 is the target of miR-663 in SKOV3 cell. a Sequence alignment of wild-type and mutant type of miR-663-binding sites in 3′-UTR of TUSC2. b Luciferase activity was reduced in SKOV3 that co-transfected miR-663 along with WT TUSC2-3′-UTR. c The mRNA level of TUSC2 in miR-663 over-expressing SKOV3 was detected by qRT-PCR method. d Immunoblotting analysis of TUSC2 expression in miR-663 over-expressing SKOV3 cells. N = 3, **P < 0.01, compared to miR-NC