Fig. 6

MiR-153-3p enhanced the tumor suppression capability of IR in vivo. U251/miR-NC or U251/miR-153-3p cells were subcutaneously injected into nude mice. The mice were exposed to IR at a dose of 8 Gy after 10 days injection. a Representative images of tumors formed in the mice in which miR-153-3p or miR-NC-infected U251 cells were implanted. b Tumor volumes were measured every 5 days after injection. c Tumors were dissected, and the tumor weights were measured on day 35 after inoculation. d Expression of miR-153-3p and BCL2 in miR-153-3p or miR-NC-expressing U251 cells-derived tumors by quantitative real-time PCR analysis. Data represent mean ± SD. e The TUNEL staining detected cell apoptosis in xenograft tumors. Blue staining represents the nucleus, and green staining indicates TUNEL-positive cells. *p < 0.05, **p < 0.01 versus miR-NC