Fig. 5
From: The deubiquitinase USP38 affects cellular functions through interacting with LSD1
Colony formation analysis. a LSD1 wild-type HCT116 cells (WT, +/+) were transfected with packaged lentiviruses of pHAGE-6tag or pHAGE-6tag-Flag-USP38, the stable USP38-expressing cell lines were screened with puromycin, and a colony formation assay was performed for 10 days. An asterisk (*) indicates p < 0.05, compared to vector-transfected cells. b LSD1 single-allele knockout (KO, +/−) HCT116 cells were transfected with packaged lentiviruses of pHAGE-6tag or pHAGE-6tag-Flag-USP38 and screened with puromycin, a colony formation assay was then performed for 13 days. Three independent experiments were performed