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Table 2 Estimated genetic parameters and Hardy–Weinberg test results in 10 microsatellites loci in three wild populations of G. pumila

From: Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)

Locus

Puyehue volcano (n = 10)

Villarrica volcano (n = 10)

P. Arenas (n = 10)

Cross-amplification

Na

Ho

He

Fis

Na

Ho

He

Fis

Na

Ho

He

Fis

G. mucronata

G. caespitosa

GP.7

1

0.00

0.00

NA

1

0.00

0.00

NA

1

0.00

0.00

NA

GP.9

1

0.00

0.00

− 0.24

2

0.20

0.19*

− 0.34

2

0.56

0.42

− 0.06*

+

GP.10

2

0.30

0.27

− 0.27

3

0.67

0.50*

− 0.10

2

0.22

0.21

− 0.27*

GP.12

2

0.00

0.18*

1.00

2

0.00

0.19*

1.00

2

0.00

0.19*

1.00*

GP.13

2

1.00

0.53*

− 0.46

4

0.60

0.59*

− 1.00

2

1.00

0.53*

− 0.46

+

GP.14

2

0.10

0.10

− 0.00

1

0.00

0.00

0.00

1

0.00

0.00

0.00

+

+

GP.15

3

0.70

0.67

0.45

3

0.22

0.52

0.13

3

0.40

0.58

0.22*

+

+

GP.16

1

0.00

0.00

1

2

0.00

0.34

1.00

2

0.00

0.51*

− 1.00

+

+

GP.17

2

1.00

0.53*

0.70

2

1.00

0.53

− 1.00

2

1.00

0.53*

0.49

+

GP.18

2

0.10

0.10

0.13

3

0.10

0.28*

− 0.03

1

0.00

0.00

0.10

+

+

  1. +, successful PCR amplification; −, unsuccessful PCR amplification
  2. Na number of alleles, Ho observed heterozygosity, He expected heterozygosity, Fis fixation index
  3. *Significant deviation from Hardy–Weinberg equilibrium (P < 0.05)