Fig. 3

Dkk3 was a target of miR-214 in NRK-52E cells. a The predictive wild-type miR-214 binding sites in the 3′UTR of Dkk3 and the corresponding mutant binding sites were displayed. b The relative luciferase activity was measured by luciferase reporter assay after NRK-52E cells were cotransfected with Dkk3-WT or Dkk3-MUT and miR-214 or miR-con. The mRNA (c) and protein (d, e) levels of DKK3 were examined by qRT-PCR and western blot in NRK-52E cells introduced with miR-214, anti-miR-214, or matched controls. Each experiment was independently repeated 3 times. *P < 0.05