Fig. 1
From: Knockdown of PAICS inhibits malignant proliferation of human breast cancer cell lines
Lentivirus-delivered shRNA targeting PAICS depletes endogenous expression in ZR-75-30 breast cancer cells. a Evaluation of the lentivirus transduction rate, which was more than 80% as estimated by fluorescence and light microscopy observations. b Quantitative analysis of PAICS knockdown efficiency in ZR-75-30 cells assessed by qRT-PCR. The β-actin gene was used as an internal control. And representative immunoblot showing PAICS knockdown efficiency determined. GAPDH was used as an internal control. Data are shown as mean ± SD (n = 3; t test). **p < 0.01; magnification, ×100