Fig. 4

The involvement of CCR6 in the chemotaxis of mouse spermatogenic cell lines (GC-1 and -2) induced by CCL20, TM4 or 15P-1 cell line in vitro. The chemotactic activity of CCL20 to GC-1 (a) and GC-2 (b), with or without the immunodepletion of anti-CCR6 antibody. The chemotactic activity of the culture supernatant of TM4 cell line to GC-1 (c) and GC-2 (d), with or without the immunodepletion of anti-CCR6 antibody. The chemotactic activity of the culture supernatant of 15P-1 cell line to GC-1 (e) and GC-2 (f), with or without the immunodepletion of anti-CCR6 antibody. Normal rabbit IgG was used as negative control. **p < 0.01 and ***p < 0.001. n = 10 in each group