Fig. 3

G007-LK specifically targets LGR5⁺ WNT-dependent intestinal stem cells. a Administration schema for G007-LK enriched chow 24-36 h after initiation of lineage tracing by IP administration of tamoxifen. b Confocal imaging of duodenal tissue in a Lgr5-EGFP-Ires-CreERT2;R26R-tdTomato mouse showing traced progeny expressing RFP (tdTomato) 7 days after initiation of lineage tracing, of which the last 6 days in the presence of G007-LK (enriched chow 100 mg G007-LK/kg). c Differential interference contrast (DIC) image of same section as in b. d Overlay of b and c. e Confocal imaging of duodenal tissue in a Lgr5-EGFP-Ires-CreERT2;R26R-tdTomato mouse showing traced progeny expressing RFP (tdTomato) 7 days after initiation of lineage tracing. The mice were fed control diet. f Differential interference contrast (DIC) image of same section as e. g Overlay of e and f. Yellow arrows indicate the tip of the villi and white arrows indicate the crypt bases