Fig. 5

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by Hyclone CM based adipogenic media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained OilRedO oil droplets. Images and graphs represent the mean quantification of siRGS2 treatment wells set relative to that of siCON treatment wells from a representative experimental set (n = 3)