Fig. 5

mRNA levels of activation-related molecules in U937 cells with 40% FFP. CD11b, Casp3 and TLR2 RT-qPCR measurements in cells stimulated 24 h solely with 40% FFP (panels a, c, e) or 40% FFP plus LPS (panels b, d, f). Bars represent means and whiskers denote standard error (mRNA levels according to the 2^−∆∆CT method). Assays were performed in triplicate. Statistical analyses were performed using the Kruskal–Wallis test with multiple comparison post-test by Dunn. Analyses were 2-tailed, and a P <  0.05 value was used for significance