Fig. 4

miR-181a knock down reduces the apoptotic effect of piceatannol on melanoma cells. (1) Cell viability assay (MTS) of WM266-4 (a) and A2058 (b) cells transfected with miR-181a or miR-181a inhibitor after piceatannol treatment; (2) Silencing of miR-181a alleviates the fall of cell apoptotic activity induced by piceatannol in WM266-4 (c) and A2058 (d) cells; (3) Inhibition of miR-181a stimulates expression dysregulation of activated caspase-3, Bax and Bcl-2 proteins in WM266-4 (e) and A2058 (f) cells. *P < 0.05, **P < 0.01 compared to normal control, ##P < 0.01