Fig. 3

Expression of CART peptide in chicken ovaries by immunohistochemistry (×400). a, d Micrograph of adjacent sections (to that depicted in b, e) were incubated with normal rabbit serum. b, e representative micrograph of section through stroma of adult hen ovary follicles were incubated with rabbit anti-CART serum. c, f, Micrograph of adjacent section (to that depicted in b, e) were incubated with rabbit anti-CART serum pre-absorbed with an excess of CART peptide. a–c, small white follicles (1–2 mm in diameter); d–f large white follicles (4–6 mm in diameter). GC granulosa cell layer, TC theca cell layer, CC Cumulus cells. a–f: Magnification 400; scale bar 20 µm