Fig. 5
Depletion of USP39 induces apoptosis of U2OS cells. U2OS cells were inoculated into 6-cm dishes at a density of 2 × 105 cells per dish for cell cycle test and 80,000 cells per dish for apoptosis after 40 h lentivirus infection. a Representative images showing Annexin V/7-ADD staining results. b Statistical data of Con U937 cells, shCon infected cells and Lv-shUSP39 infected cells. c The phosphorylation level of PARP was notably enhanced in the shUSP39 infected U2OS cells by western blot analysis (Student’s t test, n = 3). d Statistic analysis of results in western blot (Student’s t test, n = 3) ***p < 0.001