Fig. 4
From: Metabotropic glutamate receptor 5 may be involved in macrophage plasticity
Effect of mGluR5 transfection on the HMGB1 and Gal-3 secretion. RAW 264.7 cells (RAW-NT) and mGluR5-transfected macrophages (RAW-mGluR5) (5–105 cells per well) were incubated with LPS (100 ng⁄ml) or IL-10 (20 nM) for 24 h, followed by the determination of HMGB1 proteins (a) and Gal-3 (b), as described in the “Methods” section. Data represented are mean ± SEM of results from four separate experiments performed in duplicate. *P < 0.05, vs RAW-NT control cells. **P < 0.05, vs RAW-mGlur5 control cells