Fig. 4

Modulation of thymocyte subsets by GH-treatment and after contact with TEC. At the end of 24 h of contact with TEC in the presence or absence of GH, thymocytes were recovered and stained with CD4/CD8 mAbs, fixed, and analyzed by flow cytometry. In a viable thymocytes were selected and quadrants were used to individualize subsets, showing the cell percentage in each. In b bars show the absolute numbers of CD4/CD8-defined thymocyte subsets. GH induced distinct modulation in subsets, increasing CD4⁻CD8⁻, CD4⁺ and CD8⁺ subsets, and decreasing the CD4⁺CD8⁺ subset. Results are expressed as mean ± SEM (n = 6/group). *p ≤ 0.05 and **p ≤ 0.01