Fig. 2From: The p. N103K mutation of leptin (LEP) gene and severe early onset obesity in PakistanPolyacrylamide gel electrophoresis of restriction digestion of amplified region for N103K, lane 1 is marker (25 bp DNA ladder SM#1191, Thermo Scientific), lanes 2 and 4 are heterozygous parents, lane 3 is homozygous proband, and lanes 5, 6 are controls. Presence of mutation abolishes one of the seven restriction sites of MnlIBack to article page