Table 1 Primer and probe sequences used in this study
From: Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
ID | Sequences (5′–>3′) | PCR product (pb) | References |
|---|---|---|---|
RPRM (forward) | CTGCGAATTTGAACGGGGTGAGT | 137 | – |
RPRM (reverse) | GCGTAAACCGTGCAGTCACGA | 137 | – |
RPRM-M (forward) | GCGAGTGAGCGTTTAGTTC | 120 | Sato et al. [8] |
RPRM-M (reverse) | TACCTAAAACCGAATTCATCG | 120 | Sato et al. [8] |
RPRM-U (forward) | TTGTGAGTGAGTGTTTAGTTTG | 113 | Sato et al. [8] |
RPRM-U (reverse) | TAATTACCTAAAACCAAATTCATC | 113 | Sato et al. [8] |
B-actin-M (forward) | TGGTGATGGAGGAGGTTTAGTAAGT | 133 | Moon et al. [30] |
B-actin-M (reverse) | AACCAATAAAACCTACTCCTCCCTTAA | 133 | Moon et al. [30] |
RPRM (probe qMSP) | /56-FAM/TT CGC GTC G/ZEN/T TCG CGG CGT TCG TT/3IABkFQ/ | 120 | – |
B-actin (probe qMSP) | /56-FAM/AC CAC CAC C/ZEN/C AAC ACA CAA TAA CAA ACA CA/3IABkFQ/ | 133 | Moon et al. [30] |