SEMA3B is a target of miR-221. a Binding sites of miR-221 (the black letters) on the SEMA3B mRNA 3′UTR. b An inverse correlation between miR-221 and SEMA3B was observed by Spearman correlation analysis in 30 cased of human glioma tissues. c Western blot was performed to detect the protein level of SEMA3B in NHA and U87MG cells with miR-221 overexpression or knockdown using miR-221 inhibitor or mimic (100 pM) for 48 h, respectively. d Luciferase reporter plasmids and miR-221 mimics were co-transfected to cells, and then the luciferase reporter assay was employed to detect luciferase activity. *p < 0.05; **p < 0.01.