Fig. 3

Human recombinant leptin induces morphological changes in favour of angiogenesis. Haematoxylin and eosin stained histological images of CAM treated with 1, 3 and 5 μg of HRL and control with 1X PBS for 72 h. Control CAM (a) shows thin chorionic (ce) and allantoic (ae) epithelial layers with sub epithelial capillary network (SEC), CAM treated with 1 μg of HRL (b) shows comparatively thicker chorionic and allantoic epithelial layer along with small vessels at the stroma region. CAM treated with 3 μg of HRL (c) shows the presences of numerous large vessels at the surrounding of large one with increased tissue thickness at the primary stratum. CAM treated with 5 μg of HRL (d) comparatively lesser number of small vessels around the large one than CAM treated with 3 μg of HRL. Gelatin sponges has not caused wound or inflammation. Representative sections are 7 μM in thickness and were recorded at 10X magnification and bar is 50 μM. (v for vein, lbv for large blood vessel and sbv for small blood vessel)