Fig. 4

dCTCF-binding sites of the same class tend to maintain local domains. a–c The enrichment of dCTCF sites at domain boundaries. The abbreviation “12D” means regions of conserved synteny across 12 Drosophila species, and “HD” means syntenic blocks conserved between Human and D. melanogaster. The x axes represents the distance from specific dCTCF-associated boundaries. Negative and positive values indicate binding sites within and outside of domains, respectively. d–f Boxplots show the distribution of minimum domain numbers between specific dCTCF-associated boundaries. (Please notice that here we only plotted the IM/IM_random to IM boundaries, IO/IO_random to IO boundaries, and MO/MO_random to MO boundaries). The suffix “_random” indicates control sets. Boxplots are pairwise and marked by brackets. The distribution between normal and control sets are significantly different (Wilcoxon test, ** p < 1e-8, * p < 0.05)