Effects of different treatments on Cyclin D1, Bcl-2 and Survivin protein expression in Hep3B cells. (A) The protein expressions of Cyclin D1, Bcl-2 and Survivin in Hep3B cells were measured by Western blot analyses after 72 hours of siRNA transfection. β-actin was used as a housekeeping gene control. The size of each protein was indicated. (B, C, D) Densitometric analyses of these three proteins Cyclin D1 (B), Bcl-2 (C) and Survivin (D) were made relative to β-actin. Values were given as mean value ± standard deviation (SD) of triplicate. *p < 0.05 compared to untreated cell group and #p < 0.05 compared to siRNA cocktail treated cell group.