Stress tolerant virulent strains of Cronobacter sakazakii from food

Biological Research

Table 8 List of primer pair for PCR amplification

Primer	Sequence 5' to 3'	Targeted site	Product size (bp)	Reference
Esakf/	GCTYTGCTGACGAGTGGCGG^a	16S rDNA	929	[33]
Esakr	ATCTCTGCAGGATTCTCTGG	16S rDNA	929	[33]
EsgluAf/	TGAAAGCAATCGACAAGAAG^b	gluA	1680	[34]
EsgluAr	ACTCATTACCCCTCCTGATG	gluA	1680	[34]
Saka 1a/	ACAGGGAGCAGCTTGCTGC^c	V1g	952	[14]
Saka 2b	TCCCGCATCTCTGCAGGA	V3h	952	[14]
ESSF/	GGATTTAACCGTGAACTTTTCC^d	ompA	469	[35]
ESSR	CGCCAGCGATGTTAGAAGA	ompA	469	[35]
Zpx F/	GAAAGCGTATAAGCGCGATTC^e	zpx	94	[27]
Zpx R	GTTCCAGAAGGCGTTCTGGT	zpx	94	[27]

^a&cRunning conditions; 94°C for 5 min; 35 cycles of 94°C for 1 min each; 60°C for 1 min; 72°C for 1.5 min; a final extension period of 5 min at 72°C.
^bRunning conditions; 94°C for 10 min; 35 cycles of 94°C for 30 sec each; 60°C for 1 min; 72°C for1.5 min; final extension period of 5 min at 72°C.
^dRunning conditions; 94°C for 5 min; 35 cycles of 94°C for 1 min each; 54°C for 45 sec; 72°C for1.5 min; final extension period of 5 min at 72°C.
^eRunning conditions; 94°C for 15 min; 35 cycles of 94°C for 1 min each; 58°C for 1 min; 72°C for1.5 min; final extension period of 10 min at 72°C.

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