Primer
|
Sequence 5' to 3'
|
Targeted site
|
Product size (bp)
|
Reference
|
---|
Esakf/
|
GCTYTGCTGACGAGTGGCGGa
|
16S rDNA
|
929
|
[33]
|
Esakr
|
ATCTCTGCAGGATTCTCTGG
|
EsgluAf/
|
TGAAAGCAATCGACAAGAAGb
|
gluA
|
1680
|
[34]
|
EsgluAr
|
ACTCATTACCCCTCCTGATG
|
Saka 1a/
|
ACAGGGAGCAGCTTGCTGCc
|
V1g
|
952
|
[14]
|
Saka 2b
|
TCCCGCATCTCTGCAGGA
|
V3h
|
ESSF/
|
GGATTTAACCGTGAACTTTTCCd
|
ompA
|
469
|
[35]
|
ESSR
|
CGCCAGCGATGTTAGAAGA
|
Zpx F/
|
GAAAGCGTATAAGCGCGATTCe
|
zpx
|
94
|
[27]
|
Zpx R
|
GTTCCAGAAGGCGTTCTGGT
|
- a&cRunning conditions; 94°C for 5 min; 35 cycles of 94°C for 1 min each; 60°C for 1 min; 72°C for 1.5 min; a final extension period of 5 min at 72°C.
- bRunning conditions; 94°C for 10 min; 35 cycles of 94°C for 30 sec each; 60°C for 1 min; 72°C for1.5 min; final extension period of 5 min at 72°C.
- dRunning conditions; 94°C for 5 min; 35 cycles of 94°C for 1 min each; 54°C for 45 sec; 72°C for1.5 min; final extension period of 5 min at 72°C.
- eRunning conditions; 94°C for 15 min; 35 cycles of 94°C for 1 min each; 58°C for 1 min; 72°C for1.5 min; final extension period of 10 min at 72°C.