Table 2 List of oligonucleotide primers used in this study
From: Loop-mediated isothermal amplification assays for screening of bacterial integrons
Target | Sequence (5’ to 3’) | Size (bp) | Position | GenBank no. |
|---|---|---|---|---|
intI1 | Â | Â | Â | AF550415 |
F3 | AACAGTCTTGTACAAGTCCA | 20 | 903-922 | |
B3 | GGTGCTTTTGATATTTTTCCG | 21 | 1075-1095 | |
FIP | CTCTCTTTCCTCTGCGGTCCTTTTGATGTTTTTCACACTTATTGGAT | 43 | 936-958, 976-995 | |
BIP | TAAGGAATCACCTTGCAGATAAACTTTTTAGTACATTGGCATCGTGT | 43 | 1000-1024, 1057-1074 | |
LF | CCAGAGTTAAGATTGAT | 17 | 959-975 | |
LB | CGAAACAAGGCCAGTTTTTTACC | 23 | 1028-1050 | |
intI2 | Â | Â | Â | AP002527 |
F3 | TGTTGGAAGAATTTCTTTTGGA | 22 | 726-747 | |
B3 | GCTAATAGCCCTGCGTATC | 19 | 924-942 | |
FIP | CGCGATGCATGATGATGACAATTTTTAGTGTTAATGCAATTCTGGGT | 43 | 748-768, 788-809 | |
BIP | GAGCTTCCTTCTATGTGCCCGTTTTCAGAGTGGATGAGTCCCA | 39 | 832-852, 895-912 | |
LF | TCGCACCGTAATTATGACT | 19 | 769-787 | |
LB | AGATGGAAGAGTGCGTGGG | 19 | 855-873 | |
intI3 | Â | Â | Â | AY219651 |
F3 | TCGGTGTCTGTTATTAACCA | 20 | 175-194 | |
B3 | TGGAAACCGTTGTCACAC | 18 | 371-388 | |
FIP | AGACGAAGATGGTCAAAACGCTTTTGCAGTTATTTTGCTGTGGA | 40 | 206-224, 252-272 | |
BIP | CCGGGTTCGTTAATACGGCATTTTCGGGCACTGATATATGTGT | 39 | 302-321, 352-370 | |
LF | TGATAGACATCAAGCCCTCGT | 21 | 228-248 | |
LB | CAAATACTTTCTACCGTTTT | 20 | 323-342 |