Table 2 Intestinal differentiation markers and parameters used to evaluate positivity
From: CDX-2, MUC-2 and B-catenin as intestinal markers in pure mucinous carcinoma of the breast
Marker | Mark | Dilution | Positivity | Proportion | Intensity |
|---|---|---|---|---|---|
MUC 2 | Cell Marque MRQ-18 | Prediluted | Luminar/Cytoplasmic Cytoplasmic/membranous | >5% of neoplastic cells | 0 = Negative |
1 = weak | |||||
2 = moderate | |||||
3 = strong | |||||
CDX 2 | Biocare Medical CDX2-88 | 1: 50 | Nuclear | 25% = 1+ | 1 + = weak |
26-75% = 2+ | 2 + = strong | ||||
>75% = 3+ | |||||
B-catenin | Santa Cruz E-5 | 1: 50 | Membranous | 0 = 0 – 10% | 0 = Negative |
1 = 10 – 25% | 1 = weak | ||||
2 = 25 – 50% | 2 = moderate | ||||
3 = 50 – 75% | 3 = strong | ||||
4 = >75% |