Figure 5

The amount of ADAM10 at the cell surface and the proportion of cells with ADAM10 at the cell surface are stage-specific. Seminiferous tubules from adult rat testes were isolated and then dissected according to the different segments (Pale, Weak, Strong and Dark). After disaggregation, the live cells were labelled with the antibodies against ADAM10 coupled to Phycoerythrin. Then, the intensity and the percentage of positive cells were determined by flow cytometry. The figure shows (A) the histogram, (B) the percentage of fluorescence intensity (amount of ADAM10 at the cells surface), (C) the dot plot and (D) the percentage of positive cells according to the different segments. The values represent the mean ± S.E.M. of three different experiments (**p < 0.01, ***p < 0.05).