Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii

Rahmad, Norasfaliza; Al-Obaidi, Jameel R; Rashid, Noraswati Mohd Nor; Zean, Ng Boon; Yusoff, Mohd Hafis Yuswan Mohd; Shaharuddin, Nur Syahidah; Jamil, Nor Azreen Mohd; Saleh, Norihan Mohd

doi:10.1186/0717-6287-47-30

Biological Research

Table 1 Proteins identified from the differential 2D-DIGE analysis obtained from 3 different developmental stages (mycelium, primordium and fruiting bodies) of the edible mushroom Termitomyces heimii

From: Comparative proteomic analysis of different developmental stages of the edible mushroom Termitomyces heimii

Spot no	Protein name	Accession no.	^aProtein MW	Protein pI	Peptide count	Protein score	Protein score C.I%	Total ion	Total ion score C.I%	Average ratio value
Spot no	Protein name	Accession no.	^aProtein MW	Protein pI	Peptide count	Protein score	Protein score C.I%	Total ion	Total ion score C.I%	^bP/M	p value	^cF/M	p value
172	Predicted protein [Laccaria bicolor S238N-H82]	gi\|170097613	73,362	5.2	8	459	100	437	100	-1.4	0.14	-7.4	0.00057
109	NADP-glutamate dehydrogenase [Laccaria bicolor]	gi\|1101027	48,444	6	8	348	100	317	100	5.9	0.026	42.0	0.00042
106	NADP-glutamate dehydrogenase [Laccaria bicolor]	gi\|1101027	48,444	6	9	342	100	303	100	1.2	0.58	16.9	0.0021
247	Translation elongation factor 1-alpha [Uromyces polygoni-avicularis]	gi\|197726092	17,846	8.6	5	215	100	184	100	5.8	0.0014	-1.7	0.069
199	Cysteine peroxiredoxin [Laccaria bicolor S238N-H82]	gi\|170090830	25,089	5.8	5	165	100	95	100	1.7	0.12	8.2	4.50E-06
175	Carbohydrate esterase family 4 protein [Laccaria bicolor S238N-H82]	gi\|170093812	27,286	6.2	3	159	100	149	100	10.9	0.00094	3.3	0.0099
200	Cysteine peroxiredoxin [Laccaria bicolor S238N-H82]	gi\|170090830	25,089	5.8	5	145	100	104	100	1.8	0.076	33.6	1.20E-06
61	Phosphoglucomutase [Micromonaspusilla CCMP1545]	gi\|303273426	64,752	5	6	126	100	114	100	-1.2	0.76	4.1	0.0093
146	Fructose 1,6-bisphosphate aldolase [Laccaria bicolor S238N-H82]	gi\|170106499	38,916	5.5	7	126	100	98	100	1.6	0.027	12.0	9.70E-06
118	Predicted protein [Laccaria bicolor S238N-H82]	gi\|170100663	51,868	6.6	5	115	100	103	100	17.7	0.003	16.6	0.0016
126	Putative sulfite reductase [Janibacter sp. HTCC2649]	gi\|84497034	11,622	6.3	3	97	100	80	100	1.2	0.31	5.4	0.00019
131	Aspartic peptidase A1 [Laccaria bicolor S238N-H82]	gi\|170091822	44,125	5	6	86	98	66	99	9.2	0.00012	1.1	0.58
90	Hypothetical protein MPER_03344 [Moniliophthoraperniciosa FA553]	gi\|238605335	18,291	8	1	85	97	80	100	19.1	0.00056	1.2	0.59
240	DNA primase [Oceanicolagranulosus HTCC2516]	gi\|89070862	69,526	6.8	13	83	96	37	0	47.4	7.00E-07	12.6	2.90E-06
244	(p)ppGppsynthetase I SpoT/RelA [Actinobacillussuccinogenes 130Z]	gi\|152978696	70,073	8	7	71	33	55	91	1.2	0.08	47.7	7.20E-07
73	Hypothetical protein MPER_10776 [Moniliophthoraperniciosa FA553]	gi\|238582725	28,866	7.2	1	66	0	66	99	65.7	0.00014	37.3	0.00012
242	Metallophosphoesterase [Spirosomalinguale DSM 74]	gi\|284036135	26,893	5.8	1	64	-	64	99	-2.4	0.013	5.3	0.0011
85	Hypothetical protein Phum_PHUM233470 [Pediculushumanuscorporis]	gi\|242010560	222,895	9.2	14	63	-	63	99	19.9	2.80E-06	99.3	1.60E-06
173	NADH dehydrogenase (quinone) [Opitutus terrae PB90-1]	gi\|182412569	45,006	6.8	1	61	-	61	98	12.5	5.80E-05	2.0	3.10E-03

^aProtein Molecular Weight; ^bPrimordium/Mycelium; ^cFruiting bodies/Mycelium; +/-up-regulation/down-regulation.

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ISSN: 0717-6287

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