Figure 4

Cx decreases proliferation, VEGF production and promotes apoptosis of a murine A/J mammary tumor (TA3 MTXR). Tumor samples showed a decreased proliferation in Cx-treated group (B), when compared with control group (A). Relative expression is represented (C) (***: p < 0.0001) VEGF expression was reduced in treated group (E) when it was compared with control group (D). Relative expression is represented (F) (*: p = 0.0178). Apoptotic nuclei of lung sections were labeled with a TUNEL assay and treated group (H) showed an increased apoptotic nuclei/field when it was compared with control group (G). Apoptotic nuclei per field are represented (I) (***: p < 0.0001).