Fig. 6

Ex vivo treatment with anti-IL-17 reverses the increase of NF-κB activation in Purkinje neurons, glia and granular neurons. Ex vivo treatment with anti-IL-17 reversed this activation. Nuclear translocation of NF-κB in Purkinje neurons (A, F) and the increase of NF-κB in microglia (B, G), astrocytes (C, H) and granular neurons (D, I) was analyzed by immunohistochemistry with DAB staining using antibodies against NF-κB (A, D) and double fluorescence staining using anti- IBA1 (B, G) or GFAP (C, H). Representative images of the immunohistochemistry and double fluorescence staining are shown (A–D). Protein content of IKBα (E) in cerebellar slices assessed by Western blot. Representative images of the blot are shown. Values are mean ± SEM of 4–6 rats per group in A–D and 20–24 in E. One-way ANOVA followed by Fisher’s LSD post-hoc test was performed to compare all groups. Values are the mean ± SEM. Values significantly different from control group are indicated by asterisk (*p < 0.05, **p < 0.01, ****p < 0.0001) and values significantly different from HA group are indicated by a (a = p < 0.05, aa = p < 0.01, aaaa = p < 0.0001). Yellow arrows mean colocalization of the two proteins while white arrows mean non-colocalization