Fig. 4

Ex vivo treatment with anti-IL-17 reverse the alterations in the TNFα-TNFR1-S1PR2-CCL2-BDNF-TrkB pathway in cerebellar slices from hyperammonemic rats. Protein content of (A) TNFα (n = 20–30), (D) CCL2 (n = 17–27), (G) BDNF (n = 18–28) and (H) TrKB (n = 22–26) in cerebellar slices, assessed by Western blot. Membrane expression of (B) TNFR1 (n = 17–35), (C) S1PR2 (n = 19–27), (E) CCR2 (n = 20–30), (F) P2X4R (n = 12–20), (I) TrKB (n = 12–25) and (J) KCC2 (n = 16–27) was analyzed using BS3 cross-linker. Representative images of the blots of each protein are shown. Values are expressed as percentage of controls and are the mean ± SEM. One-way ANOVA followed by Fisher’s LSD post-hoc test was performed to compare all groups. Values significantly different from controls are indicated by asterisk (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and values significantly different from HA group are indicated by a (a = p < 0.05, aa = p < 0.01, aaa = p < 0.001, aaaa = p < 0.0001)