Fig. 3

Ex vivo incubation with anti-IL-17 reduces astrocytes and microglia activation in cerebellar slices from hyperammonemic rats. Representative images of immunohistochemistry against GFAP (A), IBA1 in white matter (B) and IBA1 in molecular layer (C) in cerebellar slices. GFAP stained area expressed as percentage of total area (D), the perimeter of microglia (IBA1 stained cells) in white matter (E) and molecular layer (F) are expressed in μm (n = 4–6). One-way ANOVA followed by Fisher’s LSD post-hoc test was performed to compare all groups. Values are the mean ± SEM. Values significantly different from control group are indicated by asterisk (*p < 0.05, *p < 0.01, ****p < 0.0001) and values significantly different from HA group are indicated by a (aaa = p < 0.001, aaaa = p < 0.0001)