Fig. 4

Inhibition of glutaminolysis alleviated endometrial fibrosis in the IUA mice model. The uteri of mice were divided into 4 groups: non-injured-control represented uninjured uterus from DMSO-treated mice; injured-control represented injured uterus from DMSO-treated mice; non-injured-BPTES represented uninjured uterus from BPTES-treated mice; injured-BPTES represented injured uterus from BPTES-treated mice. A Representative α-SMA and GLS1 co-immunofluorescence-stained transverse uterine sections of IUA model mice. Scale Bars = 100 μm. B Representative images of HE staining (Scale Bars = 400 μm), Masson staining (Scale Bars = 100 μm), and α-SMA IHC staining (Scale Bars = 100 μm) of uterine transverse sections from each group. C–E Quantitative analysis of the average endometrial thickness (C), the percentage of the blue areas of Masson staining (D), and the MOD of α-SMA staining (E) in each group. n = 5 in each group. Statistical analyses were performed by one-way ANOVA plus Tukey’s multiple comparisons test. F Representative images of embryo implantation numbers in each group. G Quantitative analysis of the relative embryo implantation rates. n = 5 in each group. Statistical analyses were performed by Mann Whitney test. *p < 0.05, **p < 0.01, ***p < 0.001