Fig. 2

GLS1 expression was increased in the fibrotic endometrium of IUA patients. A Representative HE staining, Masson staining, α-SMA IHC staining and GLS1 IHC staining of sections from normal endometrium and IUA samples with different stages of fibrosis (including dense fibrous lesions and borderline fibrosis lesions). Scale Bars = 200 μm. B–D Quantitative analysis of the percentage of the blue areas of Masson staining (B), and the mean optical density (MOD) of α-SMA staining (C) and GLS1 staining (D) in each group. All data were obtained from five normal endometrial patients and five IUA patients. Statistical analyses were performed by one-way ANOVA plus Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001