MicroRNA-148b secreted by bovine oviductal extracellular vesicles enhance embryo quality through BPM/TGF-beta pathway

Table 2 Kinetics of development at 48 and 96 h post-insemination (hpi) and cumulative blastocyst rates on Days 7 and 8 after IVC with or without miR-148b mimic or control mimics

	IVC N	Total cleaved N (%±SEM)	< 16 cells N (%±SEM)	≥ 16 cells N (%±SEM)	IVC 96 hpi	Blastocysts
						D7	D8
						N (%±SEM)	N (%±SEM)
Control	1001	874 (87.3 ± 0.7)	230 (23.2 ± 0.6)	644 (64.1 ± 0.7)	881	212 (24.4 ± 0.5)	274 (31.2 ± 0.6)
CMimic	1011	878 (86.9 ± 0.6)	229 (22.7 ± 0.4)	649 (64.2 ± 0.7)	891	212 (23.8 ± 0.5)	272 (30.6 ± 0.8)
miR148b	1019	891 (87.5 ± 0.4)	228 (22.5 ± 0.5)	663 (64.9 ± 0.6)	899	217 (24.2 ± 0.6)	282 (31.4 ± 0.8)
miR148b-OV	1052	917 (87.7 ± 0.5)	253 (23.1 ± 0.4)	664 (64.5 ± 0.5)	932	223 (24.4 ± 0.5)	290 (31.0 ± 0.5)
miR148b-UT	1056	927 (87.4 ± 0.5)	245 (23.3 ± 0.6)	682 (63.1 ± 0.4)	936	228 (23.9 ± 0.6)	291 (31.1 ± 0.8)

Embryos cultured from presumptive zygotes to blastocyst in SOF + 0.3% BSA (Control), SOF + 0.3% BSA supplemented with 1 µM control mimics (CMimic), or SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b) or embryos cultured from presumptive zygotes to 16- cell stage in SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b-OV), or embryos cultured from 16-cell stage to BD7 in SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b-UT). Data are the mean ± s.e.m. Within columns, different superscript letters indicate significant difference (P ≤ 0.001) between treatments

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