Fig. 3

POU5F1 is present in in vitro produced bovine blastocyst. (A) Representative fluorescence images of stained with Hoechst (blue - total cell number), POU5F1 (green) and CDX2 (red -trophectoderm cells) in BD7. Scale bar 50 μm. (B) Proportion of cells stained positive for POU5F1 markers at Day 7 blastocysts relative to the total cell number. Embryos cultured from presumptive zygotes to blastocyst in SOF + 0.3% BSA (Control), SOF + 0.3% BSA supplemented with 1 µM control mimics (CMimic), or SOF + 0.3% BSA supplemented with 1 µM of miR-148b mimics (miR148b) or embryos cultured from presumptive zygotes to 16- cell stage in SOF + 0.3% BSA supplemented with 1 µM of miR-148b mimics (miR148b-OV), or embryos cultured from 16-cell stage to blastocyst in SOF + 0.3% BSA supplemented with 1 µM of miR-148b mimics (miR148b-UT). Data are the mean ± s.e.m. Different letters indicate significant difference (P ≤ 0.05) between treatments