Fig. 4

Stromal cell proliferation and angiogenesis in mouse endometrium. (A) Representative immunofluorescent images showing the nuclear expression of Ki67 protein (green) in mouse endometrium (white arrow) at different timepoints of post-transplantation. Scale bar: 50 μm. (B) Relative Ki67 protein expression in the PBS group (blue bars) and the eMSC group (yellow bars) (n = 4). (C) Immunofluorescent staining of blood vessels in the mouse endometrium with CD31 (red, white arrows). Scale bar: 50 μm. (D) Relative number of blood vessels in the PBS group (blue bars) and the eMSC group (yellow bars) (n = 4). (E) Relative gene expression of Vegfa in the PBS group (blue bars) and the eMSC group (yellow bars) on post-operative Day 3, 7 and 14 (n = 4). Representative western blotting images (F) and quantitative analysis (G) of VEGF protein expression in the PBS group (blue bars) and the eMSC group (yellow bars) normalized to β-actin (n = 4). Results are presented as mean ± SD; *P < 0.05. Abbreviation: eMSC, endometrial mesenchymal stem cells; Vegfa, vascular endothelial growth factor A