Fig. 6

YULINK regulated cell migration and proliferation in PASMCs via PI3K-AKT signaling. A PASMCs and PAH-PASMCs with or without YULINK KD were treated with or without 20 ng/ml PDGF for 24 h for protein extraction. Cell lysates were subjected to western blot analysis for the indicated proteins. β-Actin served as an internal control. The numbers labeled below the respective blot lanes indicate the relative fold normalized with the internal control. B Representative images (magnification 200×) together with the bar graphs indicate the cell migration analysis of PASMCs, PASMCs with or without YULINK OE treated with LY294002 (10 µM), and PAH-PASMCs treated with LY294002. C A 5-day cell proliferation assay in the cells under the indicated treatments. *p < 0.05 compared to control PASMCs. ^#p < 0.05 between compared groups