Fig. 7

SYVN1 interacts with SIRT2 to promote its ubiquitylation and degradation. (A-B), The mRNA and protein expression of SIRT2 in the lung tissues were measured by real-time PCR and Western blot. (C), Western blot analysis of SIRT2 protein expression in BEAS-2B cells. (D), The cellular colocalization of SIRT2 and SYVN1 in BEAS-2B cells were examined by immunofluorescence staining. SIRT2 (red), SYVN1 (green), cell nucleus (DAPI, blue), merge (SIRT2 + SYVN1 + DAPI). (E), Immunoprecipitation and Western blot analysis of BEAS-2B cells, followed by IP with anti-SIRT2. (F), Immunoprecipitation and Western blot analysis of SIRT2 ubiquitylation in BEAS-2B cells. (G), Western blot analysis of SIRT2 protein expression in BEAS-2B cells treated with cycloheximide (CHX; 50 µg/ml) or MG132 (10 µM) at various time points. *, P < 0.05; ns, no significance