Fig. 6

ER stress is involved in the effect of SYVN1 on EMT in TGF-β1-induced BEAS-2B cells. (A), Western blot analysis of GRP78, GRP94, CHOP, p-PERK, total PERK, p-IRE1 and total IRE1 protein expression in whole cell lysates. (B), Western blot analysis of ATF6 protein expression in cell nucleus. (C), Representative immunofluorescence images of cells stained with GRP78 or CHOP. GRP78 (red), CHOP (red), cell nucleus (DAPI, blue). (D), Western blot analysis of GRP78 and CHOP protein expression in cells. (E), Western blot analysis of E-cadherin and Vimentin protein expression in cells. (F), Representative immunofluorescence staining of E-cadherin or Vimentin. E-cadherin (red), Vimentin (red), cell nucleus (DAPI, blue)