Fig. 5

SYVN1 overexpression suppresses EMT in TGF-β1-induced BEAS-2B cells. (A-B), The protein and mRNA expression of SYVN1 were measured using real-time PCR and Western blot in cells stimulated with TGF-β1 at different doses. (C-D), The protein and mRNA expression of SYVN1 were determined by real-time PCR and Western blot in cells transfected with empty vector or SYVN1-oe. Cells were transfected with empty vector or SYVN1-oe, followed by TGF-β1 (10 ng/ml) treatment. (E-F), The mRNA and protein expression of SYVN1 in cells using real-time PCR and Western blot. (G), Western blot analysis of α-SMA and Collagen I protein levels in cells. (H), Western blot analysis of E-cadherin and Vimentin protein levels in cells. I, Representative immunofluorescence staining of E-cadherin or Vimentin in cells. E-cadherin (red), Vimentin (red), cell nucleus (DAPI, blue). *, P < 0.05