Fig. 3

SYVN1 overexpression prevents OVA-induced collagen deposition and EMT. (A-B), Histological analysis of the lung sections stained with Masson. The percentage of collagen fibers were quantified. (C), Western blot analysis of TGF-β1, α-SMA, and Collagen I protein expression in the lungs. (D), Immunohistochemical examination of α-SMA expression in the lung sections. (E), Western blot analysis of E-cadherin and Vimentin protein expression in the lung tissues. (F-G), Immunofluorescence analysis of E-cadherin or Vimentin expression in the lung tissues. Epithelial cell marker E-cadherin (red), mesenchymal cell marker Vimentin (red), cell nucleus (DAPI, blue). *, P < 0.05