Fig. 7
sEV-MDA231 does not promote anchorage-independent growth of recipient BC cells. Anchorage-independent growth (clonogenic assay) was evaluated in recipient cells incubated with sEV-MDA231 previously treated or not with a lactadherin blocking antibody. 3.0–7.5 × 103 recipient cells were seeded in semisolid cell culture medium. Next day cells were treated with sEV-MDA231 (and every other condition day 1 (D1)). Another supplement was added at day 8 (D8). The number of clones formed were evaluated at day 14 (D14), visualized under inverted optic microscope. Each condition was tested in duplicates and at least 10 fields per condition were evaluated. Graph shows mean ± SEM. All data is representative of 3 independent experiments