Fig. 6

Blockade of lactadherin in sEV-MDA231 inhibits their pro-tumoroid formation potential. Different human BC cells were incubated with metastatic sEV-MDA231 pre-treated or not with a lactadherin blocking monoclonal antibody to evaluate its role in promoting spheroid/tumoroid formation. After 14 days, spheroids/tumoroids were visualized and photographed under an inverted microscope. Spheroids/tumoroids with a size ≥ 100 µm were quantified A. Representative images of spheroids/tumoroids formed in the different conditions after 14 days are shown D. The size bar corresponds to 100 µm. All data is representative of 3 independent experiments. **** p < 0.0001