Fig. 5

Blockade of lactadherin in sEV-MDA231 inhibits their pro-migration effect on recipient cells. Different human BC cells were incubated with metastatic sEV-MDA-MB-231 pre-treated or not with a lactadherin blocking monoclonal antibody to evaluate its role in their migratory capacity by a transwell migration assay. A MCF10A, B MCF7, C T47D, D ZR75 and E MDA-MB-231 recipient cells were seeded on PET-membrane Boyden transwell chambers and treated once with sEV-MDA231 (blocked or not with anti-lactadherin antibody for 16 h. After that, membranes were collected, fixed with 4% PFA and stained with DAPI. Cells into the membrane were counted in 10 fields on an inverted fluorescence microscope and then quantified. Graphs show mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001