Fig. 3

Lactadherin is differentially expressed in human BC cell lines. A Lactadherin (MFGE8) mRNA expression was measured in different human BC cell lines and in mammary epithelial breast cells through RT-qPCR. Lactadherin (MFGE8) mRNA expression was normalized against GAPDH; B Lactadherin protein was detected through WB analysis. Data was normalized against GAPDH detection for quantification; C Confocal microscopy evaluation of lactadherin in different BC cell lines. 120X, z-stack augmented images are shown. Scale bar represents 20 µm; D-F Lactadherin detection in BC cells by flow cytometry. Cell surface D and intracellular E, F histograms and quantifications are shown. The gating strategy is depicted in Additional file 1: Figure S2. mRNA A and protein B levels are shown as mean ± SEM. Images and data are representative of 3–4 independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001