Fig. 2

Lactadherin levels are associated with more aggressive BC subtypes. Analysis of TCGA data show that MFGE8 expression is higher in ER- and PR- BC patients and in more aggressive basal and normal-like PAM50 subtypes. A-D BC patients’ TCGA transcriptomic data were obtained from UCSC Xena database and MFGE8 expression were associated with ER, PR and HER2 histologic status. E–F Transcriptomic and proteomic data from another cohort of BC patients were downloaded directly from TCGA and association analyses of lactadherin levels with tumor stage E and PAM50 subtypes were performed F. G-I TCGA transcriptomic data from BC cell lines were obtained from UCSC Xena database and MFGE8 expression were associated with ER, PR and HER2 histologic status. Mann–Whitney test statistical analysis was performed A-C; Kruskall Wallis test and Dunn’s multiple comparisons correction were performed D; Group comparisons between stages I-II vs III-IV were carried out using Wilcoxon Rank Sum Test (Mann–Whitney U-test) E; Comparisons between PAM50 tumor subtypes were performed with Kruskal–Wallis test, followed by pairwise comparisons using Wilcoxon Rank Sum Test. P-value adjustment was performed using Holm’s method F; Kruskal–Wallis test statistical analysis was performed G-I