Fig. 3
From: An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm
Comet assay Olive Tail Moment (OTM) after incubation of sperm with 10 mM of Mn2+/Ca2+ (A) or 10 mM Mg2+/Ca2+ (B) for different incubation times (0 min, 2 min, 10 min, 30 min, 60 min) in non-permeabilized (−TX) and permeabilized (+TX) samples. Different letters indicate significant differences (P ≤ 0.05) between incubation times within non-permeabilized (−TX) and permeabilized (+TX) samples. Comparisons between non-permeabilized (−TX) and permeabilized (+TX) samples, or between ions (Mn2+/Ca2+ vs. Mg2+/Ca2+) are not depicted